Fig 2: Immunohistochemistry of seminal vesicle tumor (Envision, × 10, by OLYMPUS BX53). a H&E staining showed that the tumor cells were denser than interstitial cells. IHC findings showed that most tumor cells were strongly positive for calponin (b) and moderately positive for SMA (c) and desmin (d). None of the tumor cells showed Vimentin expression, while nonneoplastic endothelial cells expressed Vimentin (e). The positive rate of Ki-67 (f) was low

Fig 3: (A) Observation of colonies from strain AH7-7. (B) Gram staining of strain AH7-7 under the microscope (magnification 1000×, Olympus BX53).

Fig 4: Cyto-FISH for CDKN2a and CDK4. Cytospins of HNSCC cell lines were stained with the SPEC CDK4/CEN 12 Dual Color Probe or SPEC CDKN2A /CEN9 Dual Color Probe to check for gene specific amplification of CDKN2A and CDK4. The red spots indicate the centromers and the green spots indicate the specific gene. A readout was carried out with the fluorescence microscope Olympus BX53. Original magnification 1000×.

Fig 5: Adverse effects of CLP on mouse kidneys.Notes: (A) Images of H&E staining, PAS staining, and electron microscopy of kidney tissue 24 hours after CLP. Mouse kidney tissues were collected 24 hours after CLP and sectioned. Tissue sections were fixed and stained with H&E and PAS, which showed vacuolar degeneration and loss of brush borders (black arrows) in proximal tubular epithelial cells. Images at 400× magnification were acquired using a biological imaging microscope (BX53; Olympus Corporation, Tokyo, Japan). Tissue sections were also observed under a Hitachi H7500 electron microscope (Hitachi Ltd., Tokyo, Japan), which revealed engulfment of degraded cytoplasmic components by autophagosomes (red arrows). (B) Western blot for LC3-II, LC3-I, and P62. *P<0.05 vs the sham control. (C) Western blot for KL. *P<0.05 vs the sham control.Abbreviations: CLP, cecal ligation and puncture; EM, electron microscope; PAS, periodic acid-Schiff; d, days; h, hours.