Centrifuge 5427 R from Eppendorf

Product Specs
ItemCentrifuge 5427 R
CompanyEppendorf
Price Get Pricing
Catalog Number22620700 / 22620701 / 2262070 / 2262073 / 2262074 / 5409000136 / 5409000012
Speed Range100 – 16, 220 rpm
Maximum RCF25,000 rcf
Electrical Requirements120 V, 50/60 Hz
Dimensions(W x D x H) 31.9 x 54 x 25.4 cm (12.6 x 21.3 x 10 in)
Timer10 s to 9:59 h, with cont. mode
RefrigeratedYes
Rotor9 Rotors
QuantityEA
TypeRefrigerated Bench-top Centrifuge
Max. Capacity48 × 1.5/2.0 mL
Temperature Range-11°C to 40°C
Capacity48 x 1.5/2.0 mL tubes
Weight66.1 lb / 30 kg

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Local Office

Eppendorf

100 Crossing Boulevard

Framingham, MA 01702

United States

Phone: 1-800-645-3050

Company Profile

Website: www.eppendorf.com

International Office

Eppendorf

Barkhausenweg 1

Hamburg 22339

Germany

Phone: +49 (0)1803 255 911

Email: eppendorf@eppendorf.de

Company Profile

Website: http://www.eppendorf.com/

(1) Amino acid and mineral digestibility, bone ash, and plasma inositol is increased by including microbial phytase in diets for growing pigsJ Anim Sci BiotechnolDecember 10, 2023Liz Vanessa Lagos, Mike Richard Bedford, Hans Henrik SteinSamples were then stored at 4 °C for 30 min, centrifuged using a refrigerated centrifuge (Eppendorf Centrifuge 5427 R; Eppendorf AG, Hamburg, Germany) at 4 °C and 17,500 × g for 10 min. The supernatant was extracted using

(2) Protocol for quantitative proteomic analysis of heterogeneous adipose tissue-residing progenitor subpopulations in miceSTAR ProtocDecember 3, 2023Bo Shan, Clive S. Barker, Harry Theraulaz, Xiaoli Zhang, Yan Ping, Rana K. Gupta, Mengle Shao, Yibo WumiVac Duo concentrator/Duo pump","Genevac, UK",N/A NanoDrop,Thermo Fisher Scientific,N/A "Benchtop centrifuge, Centrifuge 5427 R","Eppendorf, Germany",N/A "Sonicator, Bioruptor water bath","Cosmo Bio Co. Ltd., Japan",N/A ThermoMixer C

(3) Bacterial Detection, Deformation, and Torque Loss on Dental Implants with Different Tapered Connections Compared with External Hexagon Connection after Thermomechanical CyclingMaterials (Basel)May 23, 2023Anselmo Agostinho Simionato, Emerson de Souza Santos, Adriana Cláudia Lapria Faria, Cássio do Nascimento, Renata Cristina Silveira Rodrigues, Ricardo Faria Ribeiroand the bristles were removed. The samples were centrifuged for 7 min at 13,200 rpm in a centrifuge (Centrifuge 5427 R, Eppendorf, Sao Paulo, Brazil) to pellet the harvested material and remove the supernatant, agitated

(4) Can salivary lactate be used as an anaerobic biomarker?PeerJMay 2, 2023Pingping Yan, Chunli Qin, Zengyin Yan, Chang Chen, Fengjing Zhangthe mixture vortexed again. After that, the 1.5 ml centrifuge tube was centrifuged with an Eppendorf Centrifuge 5427 R (Hamburg, Germany) at 4 °C and 10,000 rpm for 10 min. The supernatant was transferred to a new 1.5 ml

(5) Protocol for isolation and analysis of the leukemia stem cells in BCR-ABL-driven chronic myelogenous leukemia miceSTAR protocolsMarch 17, 2023Liu C, Lin X, Jin Y, Pan J.,Miltenyi Biotec,Cat#130-042-303 MiniMACS Separator,Miltenyi Biotec,Cat#130-042-102 MS Columns,Miltenyi Biotec,Cat#130-042-201 Centrifuge 5427 R,Eppendorf,N/A Centrifuge 5810 R,Eppendorf,N/A BD LSRFortessa Flow Cytometer,BD Biosciences,N/A BD

(6) Protocol to analyze bioenergetics in single human induced-pluripotent-stem-cell-derived kidney organoids using Seahorse XF96STAR ProtocolsMarch 1, 2023Verónica Miguel, Katharina Charlotte Reimer, Anna Katharina Galyga, Jitske Jansen, Julia Möllmann, Luisa Meyer, Rebekka K. Schneider, Rafael KramannMedical,16400122 Hemocytometer,Marienfeld,0640211 Eppendorf™ Safe-Lock Tubes,Thermo Fisher Scientific,16439009 Centrifuge 5427 R,Eppendorf,5409000010 Falcon™ 15 mL Conical Centrifuge Tubes,Falcon,10773501 Falcon™ 50 mL Conical

(7) Cell-free reconstitution of peroxisomal matrix protein import using Xenopus egg extractSTAR ProtocolsMarch 1, 2023Michael L. Skowyra, Tom A. RapoportSK-36620-09 Centrifugal concentrators,Amicon, "Centrifuge (refrigerated), model 5427 R",Eppendorf,Cat. # 5409000136 "Centrifuge rotor (swinging-bucket), model S-24-11-AT",Eppendorf,Cat. # 5409715003 Centrifuge (touchspin),Southwest

(8) Label-free quantitative proteomic analysis of serum exosomes from patients of renal anemia: The Good and the Bad of RoxadustatClinical ProteomicsDecember 1, 2022Xiaoe You, Baochun Guo, Zhen Wang, Hualin Ma, Xinzhou Zhangand after medication, 6 patients in total) were centrifuged at 4℃ for approximately 10 min at 1000g (Centrifuge 5427 R, Eppendorf) and the supernatant was transferred to new tubes and stored at −80 °C. Exosome isolation Serum

(9) Inflammation and cell-to-cell communication, two related aspects in frailtyImmunity & AgeingOctober 26, 2022Orietta Pansarasa, Maria Chiara Mimmi, Annalisa Davin, Marta Giannini, Antonio Guaita, Cristina CeredaDiscovery for cytokines analysis. The remaining plasma was the centrifuged at 20,000 × g for 1 h with Centrifuge 5427 R (Eppendorf, Italy). The pellet was washed with 0.22 μm filtered 1 × PBS and centrifuged for 1 h at 20,000

(10) Human Adipose-Derived Stromal Cells Delivered on Decellularized Muscle Improve Muscle Regeneration and Regulate RAGE and P38 MAPKBioengineering (Basel)August 30, 2022Lucas C. Olson, James T. Redden, LaStar Gilliam, Tri M. Nguyen, Josephina A. Vossen, David J. Cohen, Zvi Schwartz, Michael J. McCluretubes on ice for at least 5 min between runs. The homogenate was centrifuged at 9703 rpm (10,000× g) (Centrifuge 5427 R, Eppendorf, Hamburg, Germany) for 10 min, and the supernatant was used for Western blotting. Briefly,

(11) A Metabolomic Approach and Traditional Physical Assessments to Compare U22 Soccer Players According to Their Competitive LevelBiology (Basel)July 25, 2022João Pedro da Cruz, Fábio Neves dos Santos, Felipe Marroni Rasteiro, Anita Brum Marostegan, Fúlvia Barros Manchado-Gobatto, Claudio Alexandre Gobattowater. After vortexing (20 s), the samples were centrifuged at 14,000× g for 10 min at 4 °C (Eppendorf Centrifuge 5427 R). The upper organic phase was evaporated through manual drying using nitrogen gas (N60 AIR LIQUID, 99.9999%).

(12) Correlation between salivary dehydroepiandrosterone sulfate (DHEA-S) levels and cervical vertebral maturation in Saudi individualsThe Saudi Dental JournalJuly 1, 2022Sarah Z. Al Meshari, AlJazi H. Aldweeshof Dentistry, King Saud University. Samples were centrifuged using a centrifuge machine (Eppendorf Centrifuge 5427 R, Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany) and stored at −80 °C (Voegtline and Granger, 2014).

(13) Regulation of ACSL4-Catalyzed Lipid Peroxidation Process Resists Cisplatin OtotoxicityOxidative Medicine and Cellular LongevityMarch 20, 2022Feinan He, Xiaotong Huang, Guokun Wei, Xiaorong Lin, Weijian Zhang, Wei Zhuang, Wuhui He, Ting Zhan, Hai Hu, Haidi Yangbath for 40 min, cooled with flowing water, and centrifuged at 4000 rpm for 10 min using an Eppendorf Centrifuge 5427 R. Then, the supernatant was transferred to 96-well culture plates and the absorbance was detected at

(14) Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS)STAR ProtocolsMarch 18, 2022Nupur Sharma, Sadam H. Bhat, Gaurav Tripathi, Manisha Yadav, Babu Mathew, Vasundhra Bindal, Shvetank Sharma, Ekta Gupta, Jaswinder Singh Maras, Shiv Kumar Sarin(Blue), PTFE/White Silicone Septum",Thermo Scientific,C5000-54B Benchtop centrifuge,Eppendorf,Cat#5409000012 Sonicator,Helix Biosciences,I.T NO.#HBSNII-92 Vortex,Sigma-Aldrich,Z258423 Vacuum evaporator,Genevac,Cat#75871-454

(15) Fast quantification of extracellular vesicles levels in early breast cancer patients by Single Molecule Detection Array (SiMoA)Breast Cancer Research and TreatmentFebruary 1, 2022Carlo Morasso, Alessandra Ricciardi, Daisy Sproviero, Marta Truffi, Sara Albasini, Francesca Piccotti, Federico Sottotetti, Ludovica Mollica, Cristina Cereda, Luca Sorrentino, et al.protocol here briefly reported [31]. Platelet-free plasma was centrifuged at 20,000×g for 1 h with Centrifuge 5427 R (Eppendorf, Italy). The obtained pellet contains EVs. The pellet was washed with 0.22 µm filtered PBS

(16) The Phloem Intercalated With Xylem-Correlated 3 Receptor-Like Kinase Constitutively Interacts With Brassinosteroid Insensitive 1-Associated Receptor Kinase 1 and Is Involved in Vascular Development in ArabidopsisFrontiers in Plant ScienceJanuary 11, 2022Ke Xu, Joris Jourquin, Maria Fransiska Njo, Long Nguyen, Tom Beeckman, Ana Ibis Fernandezto a 2 ml eppendorf and centrifuged on a bench centrifuge at 21,000 g for 30 min at 4°C (Eppendorf Centrifuge 5427 R). The supernatant was transferred to a new tube and total protein was quantified with the Bradford assay. Affinity Purification

(17) Nicotine delivery and relief of craving after consumption of European JUUL e-cigarettes prior and after pod modificationScientific ReportsDecember 1, 2021Nadja Mallock, Andrea Rabenstein, Solveig Gernun, Peter Laux, Christoph Hutzler, Susanne Karch, Gabriele Koller, Frank Henkler-Stephani, Maria Kristina Parr, Oliver Pogarell, et al.ice-cold methanol were added to 50 µL plasma sample and then centrifuged at 4 °C and 14,000g for 15 min (Centrifuge 5427 R, Eppendorf, Wesseling-Berzdorf, Germany). Supernatant was diluted 1:1 with mobile phase A (see below)

(18) Unveiling the Enzymatic Degradation Process of Biobased Thiophene PolyestersFrontiers in ChemistryNovember 15, 2021Federico A. Bertolini, Michelina Soccio, Simone Weinberger, Giulia Guidotti, Massimo Gazzano, Georg M. Guebitz, Nadia Lotti, Alessandro Pellisthe ice-cold methanol precipitation protocol (volumetric ratio 1:1). The samples were centrifuged (Centrifuge 5427 R, Eppendorf AG, Hamburg, Germany) at 12,700 rpm at 4°C for 15 min. The supernatant was filtered through

(19) Expression and characterization of SARS-CoV-2 spike proteinsNature ProtocolsNovember 1, 2021Jeffrey M. Schaub, Chia-Wei Chou, Hung-Che Kuo, Kamyab Javanmardi, Ching-Lin Hsieh, Jory Goldsmith, Andrea M. DiVenere, Kevin C. Le, Daniel Wrapp, Patrick O. Byrne, et al.tubes (Beckman Coulter, Avanti J-26 XP) Microcentrifuge for 1.7 mL tubes, refrigerated (Eppendorf, Centrifuge 5427 R) Fast protein liquid chromatograph, with fraction collector (GE Healthcare, AKTA Pure 25) Scale,

(20) Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and GreenshellTM Mussel Feeding on Plasma of Ovariectomized RatsMetabolitesOctober 31, 2021Maryam Abshirini, Diana Cabrera, Karl Fraser, Parkpoom Siriarchavatana, Frances M. Wolber, Matthew R. Miller, Hong Sabrina Tian, Marlena C. Krugeradded, and the mixture was vortexed for 30 s and centrifuged for 10 min at 13,663× g at 4 °C (Eppendorf Centrifuge 5427 R, Germany). Blank samples were prepared using the same procedure, except that the plasma was replaced

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