Cultrex 3D Culture Matrix Rat Collagen I from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCultrex 3D Culture Matrix Rat Collagen I
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3447-020-01
Quantity100 mg
SpeciesRat
Molecule NameCollagen I
Brand/TrademarkR&D Systems, a Bio-Techne Brand
ApplicationsBioactivity
FormatType I Collagen provided at 5 mg/ml (Sircol Assay)
NCBI Full Gene Namecollagen type I alpha 1 chain
NCBI Gene AliasesCAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4
StorageProduct is stable for a minimum of 3 months if stored at 4 °C. Do Not Freeze. Storage Buffer: 20 mM Acetic Acid Sterility Testing: No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP XXIV Chapter 71 sterility test. No mycoplasma contamination detected by PCR

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Development of a human iPSC-derived placental barrier-on-chip modeliScienceJuly 21, 2023Agathe Lermant, Gwenaëlle Rabussier, Henriëtte L. Lanz, Lindsay Davidson, Iain M. Porter, Colin E. Murdoch,Tocris Bioscience,Cat#2939 NaHCO3,Sigma-Aldrich,Cat#S5761 Cultrex 3D Culture Matrix Rat Collagen I,R&D Systems,Cat#3447-020-01 "Fluorescein isothiocyanate-Dextran, 10 kDa",Sigma-Aldrich,Cat#FD10S "Tetramethylrhodamine isothiocyanate-Dextran,

(2) Adipogenic and SWAT cells separate from a common progenitor in human brown and white adipose depotsNature MetabolismJune 19, 2023Nagendra P. Palani, Carla Horvath, Pascal N. Timshel, Pytrik Folkertsma, Alexander G. B. Grønning, Tora I. Henriksen, Lone Peijs, Verena H. Jensen, Wenfei Sun, Naja Z. Jespersen, et al.plate (5,000 cells per well). For adipogenic cells, the wells were pre-coated with rat collagen I (3447-020-01, R&D systems) for 1 h at 37 °C in a cell culture incubator to support adherence. Seahorse extracellular

(3) Apabetalone Downregulates Fibrotic, Inflammatory and Calcific Processes in Renal Mesangial Cells and Patients with Renal ImpairmentBiomedicinesJune 8, 2023Dean Gilham, Sylwia Wasiak, Brooke D. Rakai, Li Fu, Laura M. Tsujikawa, Christopher D. Sarsons, Agostina Carestia, Kenneth Lebioda, Jan O. Johansson, Michael Sweeney, et al.prepared on ice by combining 2.42 mg/mL Cultrex rat collagen I (R&D Systems, Minneapolis, MN, USA, # 3447-020-01), 10× Minimum Essential Medium (MEM), and a solution of 0.05 N NaOH, 2.2% NaHCO3, and 200 mM HEPES pH

(4) In vitro production of infectious Plasmodium falciparum sporozoitesNatureDecember 1, 2022Eappen AG, Li T, Marquette M, Chakravarty S, Kc N, Zanghi G, Hoffman BU, Hettiarachchi H, Patil A, Abebe Y, et al.

(5) Analysis of reproducibility and robustness of OrganoPlate® 2-lane 96, a liver microphysiological system for studies of pharmacokinetics and toxicological assessment of drugsToxicology in VitroDecember 1, 2022Yuki Kato, Alicia Y. Lim, Courtney Sakolish, Alan Valdiviezo, Haley L. Moyer, Philip Hewitt, Piyush Bajaj, Gang Han, Ivan Rusyn(200 g, 3 min) and resuspended into 3.33 mg/mL collagen (Cultrex 3-D Culture Matrix Rat Collagen-I, 3447-020-01, R&D Systems; 5 mg/mL type 1 collagen, 1 M HEPES, 37 g/L sodium bicarbonate at a ratio of 4:1:1, respectively)

(6) Evaluation of Metabolism of a Defined Pesticide Mixture through Multiple In Vitro Liver ModelsToxicsSeptember 27, 2022Alan Valdiviezo, Yuki Kato, Erin S. Baker, Weihsueh A. Chiu, Ivan Rusyn(200× g, 3 min) and resuspended into 3.33 mg/mL collagen (Cultrex 3-D Culture Matrix Rat Collagen-I, 3447-020-01, R&D Systems; 5 mg/mL type 1 collagen, 1 M HEPES, 37 g/L sodium bicarbonate at a ratio of 4:1:1, respectively)

(7) Nicotinamide‐N‐methyltransferase is a promising metabolic drug target for primary and metastatic clear cell renal cell carcinomaClinical and Translational MedicineJune 8, 2022Anna Reustle et al.minced into approximately 2 × 2 mm pieces and resuspended in type I collagen gel (R&D Systems Cat# 3447‐020‐01). The tumour–collagen mixture was placed on top of a solidified collagen gel within a transwell insert

(8) The transcription factor PAX8 promotes angiogenesis in ovarian cancer through interaction with SOX17Science signalingApril 5, 2022Chaves-Moreira D, Mitchell MA, Arruza C, Rawat P, Sidoli S, Nameki R, Reddy J, Corona RI, Afeyan LK, Klein IA, et al.the 3D endothelial sprouting assay as previous described (60). Briefly, collagen type I (R&D systems: 3447-020-01) was used as the 3D scaffold. After incubation for solidification, the plate was removed from the incubator

(9) The aryl hydrocarbon receptor contributes to tissue adaptation of intestinal eosinophils in miceThe Journal of experimental medicineApril 4, 2022Diny NL, Schonfeldova B, Shapiro M, Winder ML, Varsani-Brown S, Stockinger B.37°C, 5% CO2. Eosinophil adhesion assay Plates were coated with 20 μl/96-well of rat collagen 1 (#3447-020-01; R&D Systems), Matrigel (Cultrex RGF BME Type II; #3533-005-02; R&D Systems), 0.1 mg/ml laminin (#11243217001;

(10) Deficiency of lncRNA SNHG12 impairs ischemic limb neovascularization by altering an endothelial cell cycle pathwayJCI insightJanuary 11, 2022Gross DA, Cheng HS, Zhuang R, McCoy MG, Pérez-Cremades D, Salyers Z, Wara AKMK, Haemmig S, Ryan TE, Feinberg MW.

(11) Deoxynivalenol interferes with intestinal motility via injuring the contractility of enteric smooth muscle cells: A novel hazard to the gastrointestinal tract by environmental toxinsEcotoxicology and environmental safetyAugust 16, 2021Ji X, Qiao Y, Zheng W, Jiang H, Yao W.

(12) Gamma-synuclein is a novel prognostic marker that promotes tumor cell migration in biliary tract carcinomaCancer medicineAugust 1, 2021Takemura Y, Ojima H, Oshima G, Shinoda M, Hasegawa Y, Kitago M, Yagi H, Abe Y, Hori S, Fujii-Nishimura Y, et al.Plates (Essen BioScience) were coated overnight with 300 μg/ml of 3‐D Culture Matrix Collagen I (Cat# 3447‐020‐01, R&D systems). The following day, collagen I dilution was aspirated, and cells were plated in six replicates

(13) α-Catenin levels determine direction of YAP/TAZ response to autophagy perturbationNature communicationsMarch 17, 2021Pavel M, Park SJ, Frake RA, Son SM, Manni MM, Bento CF, Renna M, Ricketts T, Menzies FM, Tanasa R, et al.

(14) 3D spheroid models of paediatric SHH medulloblastoma mimic tumour biology, drug response and metastatic disseminationScientific reportsFebruary 19, 2021Roper SJ, Linke F, Scotting PJ, Coyle B.

(15) Vav2 catalysis-dependent pathways contribute to skeletal muscle growth and metabolic homeostasisNature communicationsNovember 16, 2020Rodríguez-Fdez S, Lorenzo-Martín LF, Fernández-Pisonero I, Porteiro B, Veyrat-Durebex C, Beiroa D, Al-Massadi O, Abad A, Diéguez C, Coppari R, et al.human basic fibroblast growth factor (Cat. #hFGDBCF, Immunostep) on collagen type I-coated wells (Cat. #3447-020-01, Cultrex, R&D Systems). Activation and proliferation of satellite cells One thousand cells were plated

(16) The Microfluidic Trainer: Design, Fabrication and Validation of a Tool for Testing and Improving Manual SkillsMicromachinesSeptember 19, 2020Costa F, Falzetti L, Baldini N, Avnet S.

(17) Robust and Scalable Angiogenesis Assay of Perfused 3D Human iPSC-Derived Endothelium for Anti-Angiogenic Drug ScreeningInternational journal of molecular sciencesJuly 7, 2020van Duinen V, Stam W, Mulder E, Famili F, Reijerkerk A, Vulto P, Hankemeier T, van Zonneveld AJ.Dulbecco’s phosphate-buffered saline (dPBS, 14190-094, Thermo Scientific, USA), adjacent to a patterned collagen-1 gel (3447-020-01, R&D systems, UK). The ECs were cultured for 2 days in medium supplemented with 30 ng/mL vascular endothelial

(18) SULF1 suppresses Wnt3A-driven growth of bone metastatic prostate cancer in perlecan-modified 3D cancer-stroma-macrophage triculture modelsPloS oneJanuary 1, 2020Brasil da Costa FH, Lewis MS, Truong A, Carson DD, Farach-Carson MC.consisting of hyaluronic acid (HA) (Advanced BioMatrix, CA; GS220), 3D culture collagen I (Trevigen, MD; 3447-020-01), and recombinant human perlecan domain I (PlnDmI), prepared as described in [70]. For each Wnt3A containing

(19) Nrf2 and SQSTM1/p62 jointly contribute to mesenchymal transition and invasion in glioblastomaOncogeneDecember 1, 2019Pölönen P, Jawahar Deen A, Leinonen HM, Jyrkkänen HK, Kuosmanen S, Mononen M, Jain A, Tuomainen T, Pasonen-Seppänen S, Hartikainen JM, et al.

(20) A Role of Agrin in Maintaining the Stability of Vascular Endothelial Growth Factor Receptor-2 during Tumor AngiogenesisCell reportsJuly 23, 2019Njah K, Chakraborty S, Qiu B, Arumugam S, Raju A, Pobbati AV, Lakshmanan M, Tergaonkar V, Thibault G, Wang X, et al.

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Cultrex 3D Culture Matrix Rat Collagen I from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Cultrex 3D Culture Matrix Rat Collagen I from R&D Systems, a Bio-Techne Brand

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