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BamHI
BamHI-HF
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BamHI-HF
BamHI-HF from New England Biolabs
(1)
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Citations:
(128)
Supplier Page
Supplier Page from
New England Biolabs for
BamHI-HF
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Description
Source:
An E. coli strain that carries the cloned and modified (E163A/E167T)) BamHI gene from Bacillus amyloliquefaciens H (ATCC 49763)
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BamHI-HF
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R3136S
Concentration
20,000 units/ml
Quantity
10000 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
G⇓GATC C
C CTAG⇑G
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (128)
(1)
Reshaping the yeast galactose regulon via GPCR signaling cascade
Cell Reports Methods
December 18, 2023
Cong Fan, Jifeng Yuan
Deoxynucleotide (dNTP) Solution Mix
,
NEB
,Cat#
N0447S
BsaI-HFv2
,
NEB
,Cat#
R3733S
T4 DNA Ligase
,
NEB
,Cat#
M0202S
BamHI
,
NEB
,Cat#
R3136S
SacI
,
NEB
,Cat#
R3156S
Shrimp Alkaline Phosphatase (rSAP
),
NEB
,Cat#
M0371S
Ampicillin Na,Inalco,Cat#1758-9314
S. cerevisiae
(2)
Comprehensive identification of onco-exaptation events in bladder cancer cell lines revealed L1PA2-SYT1 as a prognosis-relevant event
iScience
December 15, 2023
Ziwei Wang, Yidie Ying, Maoyu Wang, Qing Chen, Yi Wang, Xufeng Yu, Wei He, Jing Li, Shuxiong Zeng, Chuanliang Xu
digestion of pLVX-IRES-Puro plasmids was performed using restriction enzymes
EcoRI
and
BamHI
(
R3101S
&
R3136S
,
New England BioLabs
, Massachusetts, US) according to the manufacturer’s instructions. Predicted L1PA2-
SYT1
(3)
Genome sequence and characterization of a novel Pseudomonas putida phage, MiCath
Sci Rep
December 9, 2023
James Jaryenneh, Joseph S. Schoeniger, Catherine M. Mageeney
no.
N3011S
) DNAs were digested with
EcoRI-HF
(
NEB
, catalog no.
R3101S
),
BamHI-HF
(
NEB
, catalog no.
R3136S
),
BstXI
(
NEB
, catalog no.
R0113S
),
EcoRV-HF
(
NEB
, catalog no.
R3195S
),
HaeIII
(
NEB
, catalog no.
R0108T
),
(4)
Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4
bioRxiv
November 24, 2023
Meng Yuan, Linghang Peng, Deli Huang, Amanda Gavin, Fangkun Luan, Jenny Tran, Ziqi Feng, Xueyong Zhu, Jeanne Matteson, Ian A. Wilson,
et al.
pBOBI vector that has a C-terminal FLAG tag after digestion by restriction enzymes
BamHI
and
XhoI
(
NEB
R3136S
,
R0146S
). All these plasmids were prepared with endotoxin-free kit (Takara 740426) and sterile filtered
(5)
ZIKV induction of tristetraprolin in endothelial and Sertoli cells post-transcriptionally inhibits IFNβ/λ expression and promotes ZIKV persistence
mBio
October 31, 2023
William R. Schutt, Jonas N. Conde, Megan C. Mladinich, Grace E. Himmler, Erich R. Mackow
primers found in Table S1 and inserted between pCW57-MCS1-2A-MCS2
EcoRI
(
NEB
#
R3101S
) and
BamHI
(
NEB
#
R3136S
) restriction sites.
qRT-PCR
analysis RNA was purified using RNeasy Mini Kit (Qiagen) according to
(6)
Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assay
Cell Reports Methods
October 23, 2023
Fei Zhao, Yamei Hu, Zhangling Fan, Baoying Huang, Liang Wei, Yu Xie, Yu Huang, Shan Mei, Liming Wang, Lingwa Wang,
et al.
,
Thermo Fisher Scientific
,Cat#
15070063
DMEM,Hyclone,Cat#SH30243.01 FBS,Hyclone,Cat#SH30396.02
EcoRI-HF
,
NEB
,Cat#
R3101S
BamHI-HF
,
NEB
,Cat#
R3136S
GenCRISPR™ Cas12a (Cpf1) Nuclease,GenScript,Cat#Z03502 10xNEBuffer2.1,
NEB
,Cat#
B6002S
,, Critical
(7)
Understanding cancer drug resistance with Sleeping Beauty functional genomic screens: Application to MAPK inhibition in cutaneous melanoma
iScience
October 20, 2023
Eliot Y. Zhu, Jacob L. Schillo, Sarina D. Murray, Jesse D. Riordan, Adam J. Dupuy
R0441
Enzyme:
HpaI
,
NEB
,Catalog #:
R0105S
Enzyme:
EcoNI
,
NEB
,Catalog #:
R0521S
Enzyme: BamH1,
NEB
,Catalog #:
R3136S
Enzyme: dsDNA Fragmentase,NEB,Catalog #: M0348 Enzyme:
T4 DNA polymerase
,
NEB
,Catalog #:
M0203
Enzyme:
(8)
Paired yeast one-hybrid assays to detect DNA-binding cooperativity and antagonism across transcription factors
Nature Communications
October 18, 2023
Anna Berenson, Ryan Lane, Luis F. Soto-Ugaldi, Mahir Patel, Cosmin Ciausu, Zhaorong Li, Yilin Chen, Sakshi Shah, Clarissa Santoso, Xing Liu,
et al.
converter plasmid with G418 resistance (Addgene #51680). M3926 was digested with
BamHI
(
New England Biolabs
R3136S
) and ethanol precipitated. Yα1867
yeast
were transformed with digested plasmid as follows.
Yeast
were
(9)
An Animal Model for Chronic Meningeal Inflammation and Inflammatory Demyelination of the Cerebral Cortex
International journal of molecular sciences
September 9, 2023
Enz LS, Winkler A, Wrzos C, Dasen B, Nessler S, Stadelmann C, Schaeren-Wiemers N.
(D4007, Zymo Irvine, CA, USA). The sequence for lacZ was obtained by
restriction digest
with
BamHI
(
R3136S
,
New England Biolabs
, Ipswich, MA, USA) of the plasmid LV-lacZ received as a gift from Inder Verma (Addgene
(10)
An efficient cloning method to expand vector and restriction site compatibility of Golden Gate Assembly
Cell Reports Methods
August 22, 2023
Masato Sorida, Roberto Bonasio
GeneRuler 1 kb Plus DNA Ladder
,
Thermo Scientific
,
SM1331
BsaI-HFv2
,
NEB
,
R3733S
BbsI-HF
,
NEB
,
R3539S
AgeI-HF
,
NEB
,
R3552S
BamHI-HF
,
NEB
,
R3136S
EcoRI-HF
,
NEB
,
R3101L
HindIII-HF
,
NEB
,
R3104S
NcoI-HF
,
NEB
,
R3193S
NotI-HF
,
NEB
,
R3189S
SalI-HF
,
NEB
,
R3138S
SpeI-HF
,
NEB
,
R3133S
XbaI
,
NEB
,
R0145S
XhoI
,
NEB
,
R0146S
XmaI
,
NEB
,
R0180S
KpnI-HF
,
NEB
,
R3142S
NdeI
,
NEB
,
R0111S
Smal,
NEB
,
R0141S
BbsI-HF
,
NEB
,
R3539M
T4 DNA Ligase
(11)
High-sensitive nascent transcript sequencing reveals BRD4-specific control of widespread enhancer and target gene transcription
Nat Commun
August 17, 2023
Annkatrin Bressin, Olga Jasnovidova, Mirjam Arnold, Elisabeth Altendorfer, Filip Trajkovski, Thomas A. Kratz, Joanna E. Handzlik, Denes Hnisz, Andreas Mayer
pGL3-promoter vector by either conventional restriction enzyme
cloning
using
BamHI-HF
or
SalI-HF
(
NEB
, Cat.#
R3136S
and
R3138S
) or by Gibson Assembly (
NEB
, Cat.#
E2611S
). The primer sequences used for
amplification
are
(12)
Fucosylated glycoproteins and fucosylated glycolipids play opposing roles in cholera intoxication
bioRxiv
August 3, 2023
Atossa C. Ghorashi, Andrew Boucher, Stephanie A. Archer-Hartmann, Nathan B. Murray, Rohit Sai Reddy Konada, Xunzhi Zhang, Chao Xing, Parastoo Azadi, Ulf Yrlid, Jennifer J. Kohler
encoding lentiviral vector (Addgene, plasmid no. 52962) cut with
BamHI-HF
and AgeFI (
NEB
, catalog no.
R3136S
and
R0652S
, respectively) according to manufacturer’s guidelines. Plasmids were transformed into DH5α
(13)
Long-range DNA end resection supports homologous recombination by checkpoint activation rather than extensive homology generation
eLife
June 30, 2023
Michael T Kimble, Matthew J Johnson, Mattie R Nester, Lorraine S Symington
section) other,
AatII
,
New England BioLabs
,Cat#
R0117L
,Restriction enzyme other,
BamHI-HF
,
New England BioLabs
,Cat#
R3136S
,Restriction enzyme other,
BglII
,
New England BioLabs
,Cat#
R0144S
,Restriction enzyme other,
EcoRV-HF
,
New England BioLabs
(14)
Fusion crystallization reveals the behavior of both the 1TEL crystallization chaperone and the TNK1 UBA domain
bioRxiv
June 18, 2023
Supeshala Nawarathnage, Yi Jie Tseng, Sara Soleimani, Tobin Smith, Maria J Pedroza Pedroza Romo, Wisdom Oshireku Abiodun, Christina M. Egbert, Deshan Madhusanka, Derick Bunn, Bridger Woods,
et al.
cells,
NEB
,Cat#
C2527H
,, ,, ,, "Chemicals, peptides, and recombinant proteins",,
BamHI-HF
,
NEB
,Cat#
R3136S
CutSmart Buffer
,
NEB
,Cat#
B7204S
Xhol,
NEB
,Cat#
R0146S
NdeI
,
NEB
,Cat#
R0111S
Gibson master mix,D.G.
(15)
eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content
The EMBO journal
June 15, 2023
Duan H, Zhang S, Zarai Y, Öllinger R, Wu Y, Sun L, Hu C, He Y, Tian G, Rad R,
et al
.
,
New England Biolabs
,Cat #
R0145S
XhoI
,
New England Biolabs
,Cat #
R0146S
BamHI
,
New England Biolabs
,Cat #
R3136S
T4 Polynucleotide Kinase
,
New England Biolabs
,Cat #
M0201S
T4 DNA Ligase
,
New England Biolabs
,Cat #
(16)
Mutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activity
Journal of Biological Chemistry
June 1, 2023
Michael J. Hollander, Stacy A. Malaker, Nicholas M. Riley, Idalia Perez, Nayla M. Abney, Melissa A. Gray, Julia E. Maxson, Jennifer R. Cochran, Carolyn R. Bertozzi
restriction sites. After sequence verification, plasmids were digested with
BamHI-HF
(
New England Biolabs
R3136S
)/
MfeI-HF
(
New England Biolabs
R3589S
) (gp130),
MfeI-HF
/
AgeI-HF
(
New England Biolabs
R3552S
) (LIFR),
(17)
Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodies
Cell Reports
May 30, 2023
Hailee R. Perrett, Philip J.M. Brouwer, Jonathan Hurtado, Maddy L. Newby, Lin Liu, Helena Müller-Kräuter, Sarah Müller Aguirre, Judith A. Burger, Joey H. Bouhuijs, Grace Gibson,
et al.
,
Molecular Devices
,N/A ,, Other,Other,Other ,,
PstI-HF
,
New England Biolabs
,Cat#
R3140S
BamHI-HF
,
New England Biolabs
,Cat#
R3136S
NotI-HF
,
New England Biolabs
,Cat# R3189
Q5 Site-directed mutagenesis kit
,
New England Biolabs
,Cat#
E0554S
T4 DNA ligase
(18)
AKT activation because of PTEN loss upregulates xCT via GSK3β/NRF2, leading to inhibition of ferroptosis in PTEN-mutant tumor cells
Cell Reports
May 20, 2023
Kaitlyn M. Cahuzac, Abigail Lubin, Kaitlyn Bosch, Nicole Stokes, Sarah Mense Shoenfeld, Royce Zhou, Haddy Lemon, John Asara, Ramon E. Parsons
Doxycycline hyclate
,
Sigma
,D9891-25G
Sulfasalazine
,
Sigma
,S0883 RSL3,Sigma,SML2234
Erastin
,
Sigma
,E7781 Bafilomycin,
Sigma
,
SML1661
GDC0941,
Sigma
,"957,054–30-7"
L-glutamine
,
Fisher
,MT25005CI polybrene,
Millipore Sigma
,
TR-1003-G
Blasticidin HCl,
ThermoFisher
,
A1113903
BamHI-HF
,
New England BioLabs
,
R3136S
EcoRI-HF
,
New England BioLabs
,
R3101S
MluI-HF
,
New England BioLabs
,
R3198S
BsmBI
,
New England BioLabs
,
R0739S
NotI-HF
,
New England BioLabs
(19)
Novel Antibody-independent Method to Measure Complement Deposition on Bacteria
BIO-PROTOCOL
May 5, 2023
Toksa Wonfor, Shuxian Li, Maisem Laabei
1704158
)V-bottomed 96-well plate (Thermo Fisher, Nunc, catalog number: 249570)
BamHI-HF
(
NEB
, catalog number:
R3136S
)BL21 DE3 competent cells (
NEB
, catalog number:
C2527I
). Alternatively, make your own competent cells
(20)
Probing Oxidant Effects on Superoxide Dismutase 1 Oligomeric States in Live Cells Using Single-Molecule Fluorescence Anisotropy
Chemical & Biomedical Imaging
April 24, 2023
Huanhuan Chen, Tai-Yen Chen
the mEos4b-N1 plasmid was then replaced by
PCR
-amplified mEos4b-FLAG between cutting sites
BamHI
(
NEB
R3136S
) and Notl (
NEB
R3189S
) to generate the monomeric mEos4b construct. To generate the dmE construct, we
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Reviews
Write a Review
(1)
4/16/2018
Diagnostic Restriction Mapping Of Plasmid Constructs
Triveni Mahendrappa
I have constructed a plasmid using pAYC184 as backbone with 16 kb operon making it to 20 kb size. I wanted to quickly check the constructed plasmid with restriction with BamHI and SalI double digest. ...
Read Review
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BamHI-HF from New England Biolabs
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