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AvaII (Bme18I;Eco47I;SinI)
AvaII
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AvaII
AvaII from New England Biolabs
Be the first to write a review!
Citations:
(14)
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Supplier Page from
New England Biolabs for
AvaII
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
AvaII
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0153S
Quantity
2000 units
Concentration
10-50 u/µL
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
G/G(AT)CC
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (14)
(1)
Ancient allopatry and ecological divergence act together to promote plant diversity in mountainous regions: evidence from comparative phylogeography of two genera in the Sino-Himalayan region
BMC Plant Biology
November 17, 2023
Junchu Peng, Xiangguang Ma, Hang Sun
ng) was digested by
MspI
(
New England Biolabs
,
NEB
, #
R0106L
) and
AvaII
(
New England Biolabs
,
NEB
, #
R0153L
) at 37 ℃ for 3 h in a 20 µl reaction volume buffered with 1x
CutSmart buffer
(New England Biolabs,
NEB
,
(2)
Allele specific PCR for a major marker of levamisole resistance in Haemonchus contortus
International Journal for Parasitology: Drugs and Drug Resistance
December 1, 2022
Alistair Antonopoulos, Stephen R. Doyle, David J. Bartley, Alison A. Morrison, Ray Kaplan, Sue Howell, Cedric Neveu, Valentina Busin, Eileen Devaney, Roz Laing
with the final extension at 72 °C for 5 min 20 μl of
nested PCR
product was digested using
AvaII
(
NEB
,
R0153S
) for 7–12 h at 37 °C in
CutSmart buffer
(
NEB
,
B7204S
). Digested products were then visualised on a 4%
(3)
Functional analysis of a novel de novo variant in PPP5C associated with microcephaly, seizures, and developmental delay
Molecular Genetics and Metabolism
May 1, 2022
Sara M. Fielder, Jill A. Rosenfeld, Lindsay C. Burrage, Lisa Emrick, Seema Lalani, Ruben Attali, Joshua N. Bembenek, Hieu Hoang, Dustin Baldridge, Gary A. Silverman,
et al.
variant, and were used to destroy the PAM site to prevent further editing, add a silent
AvaII
(
NEB
R0153L
) restriction site to
aid
in
genotyping
, and to introduce the proband variant. Control repair templates
(4)
Natural hybridization among three Rhododendron species (Ericaceae) revealed by morphological and genomic evidence
BMC Plant Biology
December 1, 2021
Wei Zheng, Li-Jun Yan, Kevin S. Burgess, Ya-Huang Luo, Jia-Yun Zou, Han-Tao Qin, Ji-Hua Wang, Lian-Ming Gao
al. [81]. Genomic DNA of each individual was digested with two restriction enzymes (
AvaII
,
NEB
Cat#:
R0153S
;
MspI
,
NEB
Cat#:
R0106S
), and then size-selected to a range of 500-700 bp. Library
sequencing
was performed
(5)
Lewis and ABO histo‐blood types and the secretor status of patients hospitalized with COVID ‐19 implicate a role for ABO antibodies in susceptibility to infection with SARS‐CoV ‐2
Transfusion
September 1, 2021
Eva Maria Matzhold et al.
gene was investigated by restriction fragment length polymorphism analysis using recombinant
AvaII
(
R0153S
,
New England Biolabs
, Frankfurt, Germany) for detecting the c.428G>A (rs601338) inactivating SNP.56
(6)
Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndrome
Nature communications
March 26, 2021
Inak G, Rybak-Wolf A, Lisowski P, Pentimalli TM, Jüttner R, Glažar P, Uppal K, Bottani E, Brunetti D, Secker C,
et al
.
c.530T>G mutation. For PIRA of S2, the
PCR product
of 437 bp was cut by
AvaII
(
NEB
, Ipswich, MA, USA, #
R0153S
) into 292 + 145 bp fragments only in the presence of the mutation c.769G > A. For PIRA of S2, the
PCR product
(7)
A point mutation decouples the lipid transfer activities of microsomal triglyceride transfer protein
PLoS genetics
August 1, 2020
Wilson MH, Rajan S, Danoff A, White RJ, Hensley MR, Quinlivan VH, Recacha R, Thierer JH, Tan FJ, Busch-Nentwich EM,
et al
.
site into the mutant amplicon, such that following digestion (5 units of
AvaII
(
New England BioLabs
,
R0153
) at 37˚C, 4 h) the WT band is 157 bp, homozygous mutants have bands at 129 bp and 28 bp, and heterozygotes
(8)
A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B
Human vaccines & immunotherapeutics
April 2, 2020
Ma A, Motyka B, Gutfreund K, Shi YE, George R.
5ʹ-GCATCGGACCGTACCTGCAGATCCGCAAAGGCAGAATGCGCCGCCGCCGCCAAAAGCACATATAAAACAATAGCGCTTACCATGGACCATGC-3ʹ) were synthesized and annealed together. After digestion with Ava II (New England Biolab,
R0153S
), and Rsr II (
New England Biolabs
, R051S), the fragment was cloned into Rsr II digested pFastBac-HTa,
(9)
Pigment Intensity in Dogs is Associated with a Copy Number Variant Upstream of KITLG
Genes (Basel)
January 9, 2020
Kalie Weich, Verena Affolter, Daniel York, Robert Rebhun, Robert Grahn, Angelica Kallenberg, Danika Bannasch
10042961). Prior to
PCR
, DNA was digested with New England Biosciences
AvaII
restriction enzyme (Product #
R0153L
,
NEB
, Ipswich, MA, USA). The copy number was calculated using the BioRad QX200 software standard protocol
(10)
Targetable cellular signaling events mediate vascular pathology in vascular Ehlers-Danlos syndrome
Journal of Clinical Investigation
December 17, 2019
Caitlin J. Bowen, Juan Francisco Calderón Giadrosic, Zachary Burger, Graham Rykiel, Elaine C. Davis, Mark R. Helmers, Kelly Benke, Elena Gallo MacFarlane, Harry C. Dietz
to the loss of an
AvaII
cut site and the G938D mutation leads to the gain of a
BamHI
cut site (
AvaII
R0153L
;
BamHI-HF
R3136S
,
New England Biolabs
). All
mice
found dead were assessed for
cause of death
by necropsy, noting
(11)
Modeling Niemann-Pick disease type C1 in zebrafish: a robust platform for
in vivo
screening of candidate therapeutic compounds
Disease Models & Mechanisms
September 1, 2018
Wei-Chia Tseng et al.
for 5 min.
PCR
products from both npc1y535 and npc1hg37 were digested with restriction enzyme
AvaII
(
R0153
,
New England BioLabs
, Ipswich, MA, USA) at 37°C for 8 h. Final digestion products were resolved on 2%
(12)
Using MiddRAD-seq data to develop polymorphic microsatellite markers for an endangered yew species
Plant Diversity
June 2, 2017
Hantao Qin, Guoqian Yang, Jim Provan, Jie Liu, Lianming Gao
al. (2016) was followed. Briefly, all DNA samples were double-digested with enzymes
AvaII
(
NEB
, Cat#:
R0153S
) +
MspI
(
NEB
, Cat#:
R0106S
). Barcoded adapters were annealed and ligated. Digested and ligated DNAs
(13)
Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants
Plant Methods
December 1, 2016
Guo-Qian Yang, Yun-Mei Chen, Jin-Peng Wang, Cen Guo, Lei Zhao, Xiao-Yan Wang, Ying Guo, Li Li, De-Zhu Li, Zhen-Hua Guo
were set to 600–700 bp. Reagents and enzymes used were mainly purchased from
New England Biolabs
Inc. (
R0153S
,
R0106S
),
Vazyme Biotech
Co., Ltd. (
C301-01
) and SunShineBio Co., Ltd. (SN124). Libraries were then
(14)
A streamlined tethered chromosome conformation capture protocol
BMC Genomics
December 1, 2016
Idan Gabdank, Sreejith Ramakrishnan, Anne M. Villeneuve, Andrew Z. Fire
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AvaII from New England Biolabs
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