Anti-ESPL1 antibodies are used in the immunodetection of the protein extra spindle pole bodies like 1, separase. In humans, the canonical protein has a reported length of 2120 amino acid residues and a mass of 233.2 kDa. Its subcellular localization is in the nucleus and cytoplasm. Up to 2 different isoforms have been reported for this protein. It is notably expressed in the lung and seminal vesicle. ESPL1 is reported to be a caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. Synonyms for this target antigen include SEPA, separin, caspase-like protein ESPL1, extra spindle pole bodies 1, separase, extra spindle pole bodies homolog 1, and ESP1. ESPL1 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of ESPL1 antibodies have been mentioned in research publications and have associated citations. Immunofluorescence is a widely used application for these antibodies. ELISA, Western Blot, and Immunohistochemistry are also common applications.