Anti-mitochondrial translation release factor in rescue antibodies are used for the immunodetection of the protein encoded by the MTRFR gene. In humans, the canonical protein has a reported length of 166 amino acid residues and a mass of 18.8 kDa. Its subcellular localization is in the mitochondria. Alternative splicing is reported to yield 2 different isoforms for this protein. It is reported to be expressed in all areas of the brain tested. A member of the Prokaryotic/mitochondrial release factor protein family, it is reported to be part of a mitoribosome-associated quality control pathway that prevents aberrant translation by responding to interruptions during elongation. The gene encoding this protein is implicated in Combined oxidative phosphorylation deficiency. Other names for this target antigen include probable peptide chain release factor C12orf65, mitochondrial. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.