Anti-H1-4 antibodies are used in the immunodetection of the protein H1.4 linker histone, cluster member. In humans, the canonical protein has a reported length of 219 amino acid residues and a mass of 21.9 kDa. Its subcellular localization is in the nucleus and chromosomes. It is notably widely expressed in many tissue types. A member of the Histone H1/H5 protein family, H1-4 is known to be involved with the regulation of transcription. Post-translational modifications have been described, including acetylation and phosphorylation. The H1-4 gene has been associated with the disease, Rahman syndrome. Synonyms for this target antigen include H1E, H1F4, H1s-4, HIST1H1E, RMNS, dJ221C16.5, histone H1.4, and H1.4. H1-4 gene orthologs have been reported in the mouse, rat, bovine and chimpanzee species.