Anti-Keratin 20 antibodies are used for the immunodetection of the protein encoded by the KRT20 gene. In humans, the canonical protein has a reported length of 424 amino acid residues and a mass of 48.5 kDa. Its subcellular localization is in the cytoplasm. It is reported to be highly expressed in the intestinal epithelium. A member of the Intermediate filament protein family, it is known to play a significant role in maintaining keratin filament organization in intestinal epithelia. Post-translational modifications have been described, including protein cleavage and phosphorylation.
The keratin 20 marker can be used to characterize Large Intestine Epithelial Fibroblasts, Large Intestine Crypt Goblet Cells, Large Intestine Transit Amplifying Cells, Intestinal Tuft Cells, and Colon Transit Amplifying Cells.* Other names for this target antigen include CK-20, CK20, K20, KRT21, keratin, type I cytoskeletal 20, cytokeratin 20, keratin 20, type I, and CD20. Gene orthologs have been identified in the mouse, rat, bovine and chimpanzee species. Over 140 citations in the literature describe the use of Keratin 20 antibodies in research. Western Blot is a widely used application for these antibodies. In addition, Immunohistochemistry is also a common application.
*HuBMAP Human Reference Atlas v1.4