Highly Recommended Anti-Mouse CD19 Monoclonal Antibody for Primary Cells

April 05, 2024

Immunology and Microbiology
The Scripps Research Institute
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

BUV395 Rat Anti-Mouse CD19

Catalog Number:

565965

Clone Number:

1D3

Info:Supplier Page 

Browse Similar Products: Anti-CD19 Antibody Products

We perform in vivo/in vitro targeting and engineering of mouse B cells. To analyze the on and off-target efficiency, we analyze different immune subsets. After initial gating on CD45+ cells, this CD19 antibody is used to gate on CD19+ B-cell population.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Splenocytes from C57BL/6 mouse strain

Primary Incubation

30 minutes at room temperature

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

We used 1:100 dilution of the antibody for 1 million of mouse splenocytes in 100 microliters of the total staining volume. The staining of mouse B cells was obtained and a distinct population of CD19+ cells could be gated.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific staining.

The Bad

Pricing is on higher end.

The Bottom Line

Good signal, recommended for primary cells.

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