This L1CAM Antibody From R&D Systems Was Overall Satisfactory

March 06, 2024

UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

R&D Systems

Product Name:

Anti-mouse CD171 (L1CAM) in PE

Catalog Number:

FAB5674p

Clone Number:

Lot AAYQ0222091

I use this antibody to identify L1CAM+ CD45- cells (bright) as well as L1CAM+ CD45+ immune cells (dim) in mouse lymph nodes via flow cytometry.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse lymph node cells

Primary Incubation

1:150, 30 min, 4 degrees C

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This product helps me find L1CAM+ cells in the CD45- compartment and in the CD45+ immune cell compartment. It is easy to use to identify L1CAM+ CD45- cells as they are bright (see attached picture, parent is CD45-), but you need an FMO to identify L1CAM+ CD45+ cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good separation in CD45- compartment.

The Bad

Poor separation in CD45+ compartment.

The Bottom Line

Overall, this antibody is still helpful in identifying L1CAM+ cells in mouse lymph nodes by flow cytometry. But, if you are looking for L1CAM+ immune cells, consider including an FMO sample for gating purposes.

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