Ideal Antibody to Gate Out Auto Fluorescent Eosinophils in Flow Cytometry

March 04, 2024

UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

Anti-mouse Siglec F in NovaFluor Blue 610

Catalog Number:

M042T03B06

Clone Number:

1RNM44N

Info:Supplier Page

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I use this antibody to gate out auto-fluorescent eosinophils in mouse lymph nodes to further gate and analyze the remaining immune populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymphocytes

Primary Incubation

1:800, 30 min, 4 degrees C

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This antibody is very helpful to identify and gate out eosinophils which can be very autofluorescence in flow cytometry. There is good separation with this antibody as well.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good separation, and easy to use.

The Bad

None.

The Bottom Line

I recommend this antibody to detect eosinophils via flow cytometry.

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