Need Very Little of This Anti-Mouse CD326 Antibody From BioLegend for Good Separation

UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-mouse CD326 (EpCAM) in BV711

Catalog Number:

118216

Clone Number:

G8.8

This antibody works very well for flow cytometry to identify epithelial cells as well as EpCAM positive immune cells. I dilute this antibody 1:1500 and still get really great separation of positive and negative populations!

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse epithelial cells and immune cells

Primary Incubation

1:1500, 30 min, 4 degrees C

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This product helps me identify EpCAM+ cells via flow cytometry, which includes epithelial cells as well as a small portion of EpCAM+ immune cells. It works very well and only requires 1:1500 dilution for good separation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable and small amount needed.

The Bad

Nothing significant.

The Bottom Line

Good antibody for flow cytometry.

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