Average Quantitative Elisa Kit From Eagle Bioscience

February 27, 2024

Mayo Clinic
Vaccine Research
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Eagle Bioscience

Product Name:

SARS-CoV-2 Spike Protein S2 Antigen Quantitative ELISA Assay Kit

Catalog Number:

KBVH015-18

We were interested in the detection of Spike protein in 100 people who have recovered from COVID-19 infection. The GENLISA Human SARS-CoV-2 Spike Protein S2 Antigen Quantitative ELISA kit shows promise for quantifying SARS-CoV-2 spike protein S2 antigen levels. However, inconsistent controls make replication of results challenging. The company's responsive customer service team promptly sent replacement kits when issues arose. Overall, while the kit has potential, inconsistent controls hinder its reliability.

Experimental Design and Results Summary

Application

Detection of Spike Protein in Human Serum

Starting Material

Human Serum

Tips

Thoroughly confirm controls/standards are working. Contact Company if not, they will make it right.

Results Summary

This product assisted us in quantifying trace levels of Spike protein in human sera up to 6 months after clinically documented covid infection.

DOI or PMID #

N/A

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Summary

The Good

Excellent packaging organization, and instructions.

The Bad

Poor quality control of some reagents.

The Bottom Line

This kit should be considered as it provides a fairly specific solution to many researchers. One should not hesitate to contact the supplier if anything is wrong with your results. They will likely send a replacement. After ours was replaced we used them without issue. Shipping is also very fast!

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