Anti-Mouse CD335 (Nkp46) Brilliant Violet 650™ Antibody From BioLegend Provides Good Staining

February 28, 2024

Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 650™ anti-mouse CD335 (NKp46) Antibody

Catalog Number:

137635

Clone Number:

29A1.4

This antibody provides a good NKp46 mouse staining on lymphocytes and an acceptable background. It allows good detection of mouse group 1 and group 3 innate lymphoid cells as well as NK cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse colonic cells

Primary Incubation

1/200 in PBS

Blocking Agent

Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

I use this antibody for detecting CD335 (NKp46)-positive cells among lymphocytes from mouse colon tissue. The staining was good enough and specific to perform proper gating strategy.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good and specific staining.

The Bad

The shift is just decent, you may need titration to get the best staining intensity.

The Bottom Line

Good staining of CD335 (NKp46) in lymphocytes isolated from mouse colon tissues. Great for detecting group 1 and group 3 innate lymphoid cells as well as NK cells.

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