Abcam
Anti-IRF3 antibody [IRF35I218]
ab50772
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In the SH-SY5Y cell study involving beta-amyloid or isolated mitochondria treatment, the IRF-3 antibody (#ab50772) consistently demonstrated reliability and specificity. Utilized at a 1:1000 dilution in 5% BSA TBST, it effectively identified IRF3 expression, revealing a notable decrease in the group treated with isolated mitochondria. The Western blot procedure, including overnight incubation at 4°C and a subsequent 3-hour room temperature incubation with a secondary antibody (1:1000 dilution), resulted in distinct bands. This versatile antibody proves valuable in neurodegenerative research, particularly in investigating the cGAS-STING pathway. It is advisable to use it with phosphorylated IRF-3 protein to evaluate the ratio. Adding to this, phosphorylated IRF-3 (pIRF3) represents the activated form involved in cellular responses to pathogens. To enhance specificity, it is recommended to use a different secondary antibody for pIRF3 to prevent overlap of bands and ensure accurate analysis of the IRF-3 ratio in the context of neurodegenerative processes.
Western Blot
SH-SY5Y neuronal cells
1:1000
5% BSA in TBST
N/A
Chemiluminescence
In the SH-SY5Y cell study involving beta-amyloid or isolated mitochondria treatment, the IRF-3 antibody (#ab50772) consistently demonstrated reliability and specificity. Utilized at a 1:1000 dilution in 5% BSA TBST, it effectively identified IRF3 expression, revealing a notable decrease in the group treated with isolated mitochondria. The Western blot procedure, including overnight incubation at 4°C and a subsequent 3-hour room temperature incubation with a secondary antibody (1:1000 dilution), resulted in distinct bands. This versatile antibody proves valuable in neurodegenerative research, particularly in investigating the cGAS-STING pathway. It is advisable to use it with phosphorylated IRF-3 protein to evaluate the ratio.Adding to this, phosphorylated IRF-3 (pIRF3) represents the activated form involved in cellular responses to pathogens. To enhance specificity, it is recommended to use a different secondary antibody for pIRF3 to prevent overlap of bands and ensure accurate analysis of the IRF-3 ratio in the context of neurodegenerative processes.
The bands were clear.
Perfect for western blot application.