TTC Reagent From TCl America Ensure Smooth Flow of Molecular Biology Experiments

February 09, 2024

University of South Florida
College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

TCI America

Product Name:

2,3,5-Triphenyltetrazolium Chloride or TTC

Catalog Number:

TCI-T0520

We study the CNS basement membrane and blood-brain barrier. Specifically the role of different CNS cell types in BBB maintenance.

Experimental Design and Results Summary

Application

Histology

Starting Material

Mouse Brain Slices

Tips

We use staining with 0.05% TTC.

Results Summary

2,3,5-Triphenyltetrazolium chloride (TTC) staining is a commonly used method to determine the volume of cerebral infarction in experimental stroke models.TTC is a colorless water-soluble dye that is reduced by the mitochondrial enzyme succinate dehydrogenase of living cells into a water-insoluble, light-sensitive compound (formazan) that turns healthy/normal tissue deep red.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

TTC staining is a simple, rapid, and inexpensive method that allows to delineate the size of the cerebral infarct area . TTC‐stained brain tissue can be used for molecular biology experiments without any restrictions.

The Bad

Nothing.

The Bottom Line

Simple, cost-effective, and offers a rapid visualization.

Join the discussion