Easy and Fast Way to Isolate RNA Using PureLink™ RNA Mini Kit From ThermoFisher

January 11, 2024

State University of New York at Buffalo
Physiology and Biophysics
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Thermo Fisher Scientific

Product Name:

PureLink RNA Mini Kit

Catalog Number:

12183025

We use the PureLink™ RNA Mini Kit from ThermoFisher to isolate RNA from primary mouse astrocytes for subsequent RNA-seq or qRT-PCR.

Experimental Design and Results Summary

Application

RNA extraction

Starting Material

Primary mouse astrocytes

Tips

Additionally needed: 2-mercaptoethanol and ethanol.

Results Summary

We use a column-based PureLink RNA Mini Kit to extract RNA from mouse primary astrocytes for gene expression analysis. We use an 18-gauge needle with an RNase-free syringe to homogenize the cells and follow the manufacturer's protocol without any modifications. Image: RNA profile on the TapeStation.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Fast and easy RNA extraction.

The Bad

This kit does not work with isolated cells using columns in combination with magnetic antibodies (MicroBead-labeled cells).

The Bottom Line

High-quality total RNA can be obtained in a very fast time, approximately 20 minutes. The manufacturer protocol is very well-written and does not require any special equipment (except a microcentrifuge capable of centrifuging at 12,000 x g) or toxic reagents.

Join the discussion