Viability Assay Kit From Promega to Evaluate Cytotoxicity in Spheroids

Università degli Studi Milano-Bicocca
Industrial Biotechnologies
Graduate Student

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Company:

Promega

Product Name:

CellTiter-Glo® 3D

Catalog Number:

G9681

CellTiter-Glo® 3D has been used in a viability assay to evaluate the cytotoxicity of lipidic nanoparticles loaded with drugs on human osteosarcoma spheroids. The spheroids were treated with nanoparticles to have different final drug concentrations, after 24h the assay was executed.

Experimental Design and Results Summary

Application

Viability assay for cytotoxicity

Starting Material

Human osteosarcoma cell spheroids

Tips

N/A

Results Summary

This product allowed me to evaluate the percentage of cells viability in a 3D culture and the level of drug cytotoxicity

DOI or PMID #

N/A

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Summary

The Good

Quick and easy implementation .

The Bad

Lack of accuracy and reliability.

The Bottom Line

Really quick and easy assay, only 30 minutes to have a decent idea of the cells viability.

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