Good Reagent, Slight Toxicity Depending on Dilution

UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CellTrace™ Violet Cell Proliferation Kit, for flow cytometry

Catalog Number:

C34557

Used to assay T cell proliferation in response to stimulus. The reagent works well and I can see nice peaks representing division. This reagent is slightly more toxic than other versions I have used, but changing dilution fixes it in my hands. Overall, very good reagent, others in different channels are better due to toxicity.

Experimental Design and Results Summary

Application

T cell proliferation

Starting Material

Tips

Used at 1:3000 dilution

Results Summary

Able to see the result of different stimuli on T cell proliferation. In response to stimulus, T cell populations proliferate and dilute out the dye resulting in distinct populations arising by flow cytometry.

DOI or PMID #

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Summary

The Good

Good data

The Bad

Requires some optimization due to toxicity

The Bottom Line

Works great after some optimization. Very easy to use

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