BioLegend
Spark Violet™ 538 anti-human CD3 Antibody
300484
UCHT1
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This antibody has been used to optimize flow cytometry-based immunophenotyping of PBMC samples. The results have not yet been published. Multi-colour antibody panels were designed to identify several lymphocyte populations and tested to ensure appropriate spectral unmixing was possible in a 20+ color panel.
Flow cytometry
Human PBMC
5µL Spark Violet™ 538 CD3 for 30 minutes at 4°C
Human TruStain FcX™ (Biolegend 422302) and CellBlox™ Plus (Thermo Fisher C001T06F01)
N/A
3L Cytek Aurora, Kaluza Software
Staining allowed the distinction of CD3+ T cells. Titration of the antibody was conducted, although this resulted in loss of separation between positive and negative populations. This antibody fit well into a 20+ colour panel with no unmixing issues.
Distinct staining allowing selection of T cells.
Titration resulted in loss of distinct populations.
Good antibody that provides sufficient population definition in a large multi-colour panel.