Novus Biologicals
Influenza A H1N1 Antibody - (A/California/14/2009)
NBP1-30320
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The cell line was infected with mock or test viral particles (10 moi) and after 36 hours of incubation in complete media the cell extracts were prepared, proteins were resolved in SDS-PAGE and immunoblotted with Influenza A H1N1 Antibody - (A/California/14/2009) antibody. The antibody was purchased from Novas Biological by its merit mentioned in the antibody flyer and referral from published articles and reviews.
Western blot
Total extract from virus infected and non-infected cells
1:1000 in BSA (5%), overnight at 4 degrees celsius
BSA (5%), 2h at RT
Goat-anti rabbit IgG-HRP (1:4000), 2h at RT
None
ECL
The antibody showed high specificity to HA, NA and M/NP as the bands for these viral protein markers were detected at the expected molecular mass. However, antibodies little bit cross reacted with other non-specific proteins. There was a change in the levels of these proteins in the mock and test virus infected group.
N/A
Its specificity to detect HA, NA and M/NP.
None.
Shows good sensitivity. I highly recommend this antibody.