Santa Cruz Biotechnology, Inc
Influenza A NP Antibody
sc-101352
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The cell line was infected with mock or test viral particles (10 moi) and after 36 hours of incubation in complete media a specific treatment was given and the cell extracts were prepared, proteins were resolved in SDS-PAGE and immunoblotted with influenza A NP antibody. The antibody was purchased from Santacruz Biotechnology by reading its datasheet published on the manufacturer's website.
Western Blot
Total extract from virus infected and non-infected cells
1:500 in skimmed milk (5%), overnight at 4 degrees Celsius
Skim milk (5%), 2h at RT
Goat-anti mouse IgG-HRP (1:4000), 1h at RT
None
ECL
The antibody has detected influenza A NP protein with high specificity. However, antibodies also recognized other non-specific proteins and was an increase of influenza A NP levels in virus infected cells as compared to cells that were infected with mock virus.
N/A
Its specificity to detect influenza A NP protein.
The antibody also detected several non-specific proteins.
Overall, this is an average antibody for the detection of influenza A NP.