ThermoFisher Scientific
CD276 (B7-H3) Monoclonal Antibody (M3.2D7), PE, eBioscience™
12-5973-82
M3.2D7
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I used this antibody to attempt to determine B7H3 expression in a mouse tumor cell line and did not see any signal. Unfortunately, this particular clone did not work for my cell line, and was confirmed by an isotype control.
Flow cytometry
Mouse neuroblastoma cell line
30 minutes at 4 degrees C in the dark
None
This particular clone did not work in determining B7H3 expression in my mouse tumor cell lines. This was validated by an isotype control, but I was able to identify another clone that worked for my tumor cell line.
N/A
None.
Did not work and it is expensive.
Did not work for me, but may be specific for other mouse cells.