CD64 Antibody From BioLegend for Flow Cytometric Differentiation of Alveolar Macrophages

Results Summary

I used this product confirm that my gated population was alveolar macrophages, in mice treated with PBS or clodronate liposomes. Cells were isolated from BALF using 1 ml of 1% BSA in PBS and from lungs through collagenase digestion; RBC lysis was performed using ACK buffer. Cells were then stained with viability dye followed by extracellular antibodies such as CD11b, CD11c, Siglec-F, CD64. The alveolar macrophage pool looked very clear from the live cells. Using CD11c and CD11b, alveolar macrophages were distinguished from eosinophils. CD64 expression on these cells was then confirmed as shown in the picture. I have not seen any spillover issues with this antibody and fluorophore combination, despite alveolar macrophages being auto fluorescent.