Antibody for E-Cadherin (WB) From Abcam Works Well

September 08, 2023

Pathology
Sao Paulo State University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-E Cadherin antibody

Catalog Number:

ab15148

Clone Number:

N/A

This antibody was used in a study that investigated the induction of EMT in amnion epithelial cells (AEC) in response to inflammation and infection associated with spontaneous preterm birth (SPTB)

Experimental Design and Results Summary

Applications

Western blot

Sample

Human amnion epithelial cells

Primary Incubation

1:500 dilution, overnight at 4 degrees

Blocking Agent

5% nonfat milk in 1× Tris-buffered saline-Tween 20 (TBS-T)

Secondary Incubation

1:10000, 2 hours, RT

Tertiary Incubation

N/A

Detection

FACSCalibur (Becton Dickinson), software CellQuest version 4

Results Summary

This antibody worked well for Ecad detection by WB analysis. The results look good using the 1:500 dilution. Only minimal background.

DOI or PMID #

10.1530/REP-20-0283

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good quality.

The Bad

N/A

The Bottom Line

Appropriate results for this marker.

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