Invitrogen
T-bet Monoclonal Antibody (eBio4B10 (4B10)), PE-Cyanine7, eBioscience
25-5825-82
4B10
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To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mice were stained with FITC anti-CD161 Ab and Zombie NIR in 100 µL PBS at 4C for 30 min. Subsequently, the cells were fixed/permed using eBioscience™ Foxp3 / TF Staining Buffer Set according to the manufacturer's instruction, then stained with PE-Cy7 anti-T-bet Ab in 100 µL Perm buffer at 4C for 30 min.
Flow cytometry
Splenocytes from C57BL/6 mouse
See description
N/A
BD LSRFortessa
To titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mouse were stained with: FITC anti-CD161 Ab and Zombie NIR in 100 μL PBS at 4C for 30 min. Subsequently, the cells were fixed/permed using eBioscience™ Foxp3 / TF Staining Buffer Set according to manufacturer's instruction, then stained with: PE-Cy7 anti-T-bet Ab in 100 μL Perm buffer at 4C for 30 min.
Mediocre resolution.
According to the titration, 1:200 dilution has the best SI.