Highly Sensitive ECL Western Blotting Substrate From ThermoFisher

July 29, 2023

Stanford University
Biochemistry
Postdoctoral Researcher

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Company:

Thermo Scientific™

Product Name:

Pierce™ ECL Western Blotting Substrate

Catalog Number:

32109

As a protein biochemist studying protein levels and protein-protein interactions, Western blotting is an indispensable technique for my research. It offers a sensitive and reliable means of detecting and quantifying proteins, allowing me to analyze their expression levels and investigate interactions with other molecules. The chemiluminescent properties of the ECL Western Blotting Substrate enable precise visualization of even low-abundance proteins, providing valuable insights into cellular processes and protein function.

Experimental Design and Results Summary

Application

Western blot

Starting Material

Transferred protein on nitrocellulose/PVDF membranes bound to antibodies with HRP tag.

Tips

Optimize exposure time when using ECL Western Blotting Substrate for the best signal-to-noise ratio in protein detection. Remember to standardize your protein loading and use appropriate controls to ensure accurate and reliable results in Western blot experiments.

Results Summary

In this Western blot analysis using α-tubulin as the target protein, the ECL Western Blotting Substrate proved to be instrumental in achieving high sensitivity. The substrate facilitated precise detection of α-tubulin levels, allowing for accurate quantification and visualization of the protein band.

DOI or PMID #

N/A

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Summary

The Good

Its chemiluminescent properties provide a wide dynamic range for visualizing both high and low abundance proteins, making it a valuable tool for protein biochemists studying protein levels and interactions.

The Bad

None.

The Bottom Line

High sensitivity for accurate protein detection.

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