Novus Biologicals
CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free
NBP2-36440
7A9-3A3
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The WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying the molecular mechanism of oncogenic WNT signal transduction to identify potential targets for therapeutic intervention of colon cancer. The goal of this experiment is to determine expression levels of Cas9 fusion proteins for future CRISPRi experiments.
Western blot
HEK293T stable expression using lentivirus expression system.
0.5 µg/mL for 16 h at 4 deg C
5% milk in TBS-T (0.1% Tween-20)
Donkey anti-Mouse IgG HRP at 1:10,000 dilution for 1 h at RT
None
ECL Regular
KRAB-dCas9 and dCas9-KRAB-MeCP2 were stably expressed in HEK293T cells. 50 µg of whole cell extracts were resolved on a 4-12% Bis-Tris gel and proteins were transferred onto a nitrocellulose membrane. This antibody detects stably expressed Cas9 fusion proteins.
N/A
No optimization was required.
None.
Good for Western blotting.