Cell Signaling Technology
PTEN Antibody #9552
9552
N/A
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Cell lines from syngeneic mouse models were PTEN(-/-) deletion. The immunoblotting procedure is described below. Lyse cells by adding RIPA (with Proteinase and Phosphatase inhibitors) buffer. Heat 20µg of protein to 95–100°C for 5 min. Load 20µg of protein onto SDS-PAGE gel. Electrotransfer to PVDF membrane (Semi-dry, 20V, 1 and half hours). Incubate membrane in blocking buffer (1X TBST with 5% nonfat dry milk) for 1 hour at room temperature. Incubate membrane with diluted (1:1,000) primary antibody in 5% BSA, 1X TBS, 0.1% Tween20 at 4°C with gentle shaking, overnight. Incubate membrane with the Rabbit-specific HRP-conjugated secondary antibody in blocking buffer with gentle agitation for 1 hour at room temperature.
Western blot
Syngeneic mouse models
Incubate membrane with diluted (1:1,000) primary antibody in 5% BSA, 1X TBS, 0.1% Tween20 at 4°C with gentle shaking, overnight.
1X TBST with 5% nonfat dry milk
Rabbit-specific HRP-conjugated secondary antibody in blocking buffer with gentle agitation for 1 hour at room temperature
None
SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Cell lines from syngeneic mouse models were PTEN (-/-) deletion. The immunoblotting procedure is described below. Lyse cells by adding RIPA (with Proteinase and Phosphatase inhibitors) buffer. Heat 20µg of protein to 95–100°C for 5 min. Load 20µg of protein onto SDS-PAGE gel. Electrotransfer to PVDF membrane (Semi-dry, 20V, 1 and half hours). Incubate membrane in blocking buffer (1X TBST with 5% nonfat dry milk) for 1 hour at room temperature. Incubate membrane with diluted (1:1,000) primary antibody in 5% BSA, 1X TBS, 0.1% Tween20 at 4°C with gentle shaking, overnight. Incubate membrane with the Rabbit-specific HRP-conjugated secondary antibody in blocking buffer with gentle agitation for 1 hour at room temperature.
High Sensitivity.
A weak non-specific band for mouse samples.
For PTEN deletion detection in mouse samples.