Decent Myc Antibody for Western Blot

July 11, 2023

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Mouse Anti-Myc (9E10)

Catalog Number:

sc-40 X

Clone Number:

N/A

My research involves studying compartmentalized dopaminergic signaling. We overexpress proteins of interest, tagged with markers such as Myc, and verify the expression of constructs by Western Blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HeLa and HEK293 cell lysates

Primary Incubation

1:1000, overnight, 4°C on shaker

Blocking Agent

3% milk in TBS-Tween

Secondary Incubation

1:10000, 1hr, RT on shaker

Tertiary Incubation

N/A

Detection

ECL then film/image dock

Results Summary

This helped us confirm, that our protein of interest was expressed in our cells when we performed our experiment.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clean, works well.

The Bad

Expensive.

The Bottom Line

Great product but expensive.

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