Quick Blocking of Nitrocellulose or PVDF Membranes

Dana-Farber Cancer Institute
CIV
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Thermo Fisher Scientific

Product Name:

Pierce™ Fast Blocking Buffer

Catalog Number:

37576

This blocking buffer can block nitrocellulose or PVDF membranes in only five minutes, which makes the western blot process much faster (saves ~30-60 minutes).

Experimental Design and Results Summary

Application

Blocking of nitrocellulose or PVDF membranes.

Starting Material

N/A

Tips

This blocking buffer probably can be recovered for couple of times (haven't test that yet).

Results Summary

After transferring of pritein into the nitrocellulose or PVDF membrane, incubate the membrane in the buffer for 5-10 minutes.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Time saver.

The Bad

Expensive.

The Bottom Line

It general, it saves so much time and is worthy!

Join the discussion