Glacial Acetic Acid 100%, Water Free

June 30, 2023

MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

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Company:

MilliporeSigma

Product Name:

Acetic Acid (glacial), 100%

Catalog Number:

1000660250

We bought the Glacial acetic acid because it is 100% water-free acid, so it is more concentrated. I used the reagent within the Sulforhodamine B (SRB) assay, used for cell density determination. The dye is removed by washing repeatedly with 1% (vol/vol) Acetic acid. It always works well.

Experimental Design and Results Summary

Application

Cell viability assay - dye washing steps.

Starting Material

Fixed cells stained with the dye Sulforhodamine B (SRB).

Tips

Store at room temperature > 17 °C - 25°C (lower temperatures can freeze the solution).

Results Summary

We bought the Glacial acetic acid because it is 100% water-free acid, so it is more concentrated. I used the reagent within the Sulforhodamine B (SRB) assay, used for cell density determination. The dye is removed by washing repeatedly with 1% (vol/vol) Acetic acid. It always works well.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Concentrated acetic acid.

The Bad

Can freeze at lower temperature. (~17°C)

The Bottom Line

We bought the Glacial acetic acid because it is 100 % water free acid so it is more concentrated.

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