Antibody Stains CD21/35 Very Well

Stanford

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 711™ anti-mouse CD21/CD35 (CR2/CR1) Antibody

Catalog Number:

123435

Clone Number:

7E9

We wanted to assess how certain perturbations would affect B cells in the spleen, so we used the antibody to identify CD21/35+ B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen

Primary Incubation

1:100

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT cytometer

Results Summary

We stained mouse spleen cells with the anti-CD21/35antibody and found that it identifies CD21/35+ B cells very well. The antibody was easy to use and the staining was very clear.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works great for staining CD21/35.

The Bad

None to mention.

The Bottom Line

The antibody is great for identifying CD21/35+ B cells very clearly.

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