Not Recommended CD196 Antibody for Flow Cytometry of Murine Lymphocytes

April 17, 2023

UCSF
Graduate Student

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Quality of Results

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Company:

BioLegend

Product Name:

Antimouse CD196 (CCR6) in BV605

Catalog Number:

129819

Clone Number:

29-2L17

I used this antibody in spectral flow cytometry to identify CCR6+ and CCR6- murine lymphocytes. It has poor signal and horrible separation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lymphocytes

Primary Incubation

1:300, 4C for 20 min

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

I use this antibody to detect CCR6 positivity in murine lymphocytes by spectral flow cytometry and it works very poorly.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

None

The Bad

Poor signal, horrible separation.

The Bottom Line

I do not recommend this antibody for flow cytometry staining of murine lymphocytes.

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