Good Antibody to Mark Murine NK Cells

April 17, 2023

UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-mouse NK1.1 antibody in APC-Cy7

Catalog Number:

108724

Clone Number:

PK136

Info:Supplier Page View Product Specs

Browse Similar Products: NK1.1 Antibodies

I use this antibody in all of my flow cytometry panels to mark NK cells in murine lymphoid tissue. Good staining and good separation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lymphocytes

Primary Incubation

1:300, 4C for 20 minutes

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This antibody is very good for distinguishing NK1.1+ versus NK1.1- subpopulations within murine lymphocytes by flow cytometry. It has good separation allowing for easy identification of NK cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable signal and good separation.

The Bad

None

The Bottom Line

I recommend this antibody for marking NK cells in murine tissue by flow cytometry.

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