Novus Biologicals
58K Golgi Protein Antibody
NB600-412
58K-9
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Primary mouse brain endothelial cells were stained with 58K Golgi protein antibody to differentiate secreted proteins in Golgi apparatus and cytoplasm.
Immunofluorescence
Mouse brain endothelial cells
1:200 in PBS + 0.01% Triton-X100, overnight at 4C
PBS + 0.01% Triton-X100 + 10% BSA
Mouse anti-Goat IgG AF594 1:500 :500 in PBS+0.1% Triton-X100, 2hr at 25C
N/A
Epifluorescence microscopy
Staining with 58K antibody was evident throughout the entire endothelial cell. Signal was higher at the perinuclear area in some cells but still present in the cytoplasm.
Inexpensive.
High background, low specificity.
This antibody did not adequately label Golgi apparatus in mouse brain endothelial cells. While perinuclear staining was evident, background staining was substantial.