Protein-G Agarose Beads Works Well

Results Summary

Upstate Biotechnology’s Chromatin Immunoprecipitation (ChIP) Assay Kit now has the cat # 17-295 since it was merged by Sigma-Aldrich. Chromatin is comprised of nucleosome subunits, each of which consists of DNA wound around several histone proteins. Large multiprotein complexes are often involved in regulatory processes taking place in the nucleus. The regulation of transcription in eukaryotic cells is critically dependent on the dynamic state of chromatin. Transcriptional activation and inactivation is intimately associated with the either the relaxed or taut conformation of chromatin structure. To reveal the structure and dynamics of these nuclear protein complexes to determine their interactions with the DNA template, the chromatin immunoprecipitation (ChIP) assay was developed by Upstate Biotechnology, Inc. The ChIP assay combines two straightforward steps: 1) in vivo formaldehyde cross-linking of whole cells that freezes protein-protein and protein-DNA interactions, followed by immunoprecipitation of protein-DNA complexes with specific antibodies from sonicated extracts. The kit is a collection of several key reagents (ten in total) that assist in various points during the ChIP protocol. Chromatin immunoprecipitation (ChIP) assay by ChIP-qPCR (low) and in agarose gel (upper) (n=5) in Figure 5D showed TGF-β1 signals are identified as Foxp1 direct downstream target genes in ECs to regulate the AngII-induced pathological cardiac fibrosis and control cardiac function.