Good Actin Antibody for Western Blot, but not Confocal

February 21, 2023

Houston Methodist Research Institute

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-alpha smooth muscle Actin antibody [EPR5368]

Catalog Number:

ab124964

Clone Number:

N/A

I tested this antibody on MRC-5 (human fibroblast cell lines) stimulated with TGF-b and treated with mitochondria. I got great results for WB, but I had some backgrounds for the confocal.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Fibroblast

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I tested this antibody on MRC-5 (human fibroblast cell lines) stimulated with TGF-b and treated with mitochondria. I got great results for WB, but I had some backgrounds for the confocal.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

Not good for the confocal.

The Bottom Line

Recommended for western blot.

Share your experience with other scientists. Write a Review! »

Join the discussion