Quality Anti-DCTN1/p150-glued Antibody for Western Blot

December 19, 2022

Pharmacology
PennState College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-DCTN1/p150-glued antibody

Catalog Number:

ab246505

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: p150 Antibodies

We used this antibody to detect DCTN1/p150 expression in the COS7 cell line by western blot analysis. The antibody worked well at the recommended dilution. Specificity is very good. Overall it's a good product.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mammalian cell line whole cell lysate

Primary Incubation

1:1000, overnight incubation at 4 degree Celsius

Blocking Agent

5% BSA in TBST

Secondary Incubation

Goat anti-rabbit IgG HRP Ab, dilution (1:2500) in 5% BSA , 1hr at RT

Tertiary Incubation

N/A

Detection

ECL

Results Summary

This antibody perfectly detected DCTN1/p150 expression in western blot analysis. We repeated the experiment and the results are consistent.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good specificity, less optimization and strong signal.

The Bad

N/A

The Bottom Line

I recommend this antibody for the detection of DCTN1/p150 levels.

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