Novus Biologicals
Methylglyoxal Antibody (9E7) [Alexa Fluor® 488]
NBP2-59368AF488
9E7
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This antibody worked well at a 1:100 dilution. I included a picture with an FMO and methylglyoxal treated cells as a positive control.
Flow Cytometry
T cells
1:100. 1 hr
None
Flow cytometry
I used the foxp3/perm kit and had great results at 1:100 dilution. The FMO was included as a negative control and MGO treated cells were a positive control.
N/A
Reliable and good signal.
It worked and was validated by my positive and negative controls.