R&D Systems
mNephrin
AF3159
N/A
Info:Supplier Page View Product Specs
Browse Similar Products: Nephrin Antibodies
We work on kidney and renal podocytes development. The podocytes marker Nephrin (NPHS1) is crucial to identify and follow the maturation and functionality of this cell population. This antibody showed very good staining in different conditions and tissue origins.
Immunofluorescence
Mouse renal samples
1:200 in blocking solution, 1 hour at RT
PBS + donkey normal serum 10%
Fluorescence coupled secondary antibody, 1:500 for 1 hour at RT
None
Fluorescence/confocal microscopy
Very strong and specific staining in mouse kidney slides, even using secondary antibodies know to have high background in this tissue (ie Alexa488 or Alexa555). It works in frozen tissue (OCT embedded) as well in paraffine embedded samples. It stands all the unmasking condition and pH we tested so far.
Specific and strong signal, works in all the conditions we tested.
Excellent antibody, essential for the studies focused on kidney and renal podocytes.