Good Phospho-AMPK Alpha antibody for Western Blot

October 13, 2022

Pharmacology
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-AMPKα (Thr172)

Catalog Number:

#50081

Clone Number:

D4D6D

To check whether IL-6 gene transfer affects Phospho-AMPK alpha expression, the results showed antibody detected Phospho-AMPKa expression and IL-6 enhanced Phospho-AMPKa protein level in obese mice.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mice liver tissue

Primary Incubation

1:1000 overnight at 4 degree

Blocking Agent

5% milk

Secondary Incubation

1:3000 1 hour at room temperature

Tertiary Incubation

1 minute at room temperature

Detection

ECL

Results Summary

To check whether IL-6 gene transfer affect Phospho-AMPK alpha expression, the results showed antibody detected Phospho-AMPKα expression and IL-6 enhanced Phospho-AMPKα protein level in obese mice.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good for western blot.

The Bad

N/A

The Bottom Line

Good signal and recommended.

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