Phospho-Sr Antibody Works Well

October 03, 2022

Pharmacology
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-Src Family (Tyr416) Antibody

Catalog Number:

#2101

Clone Number:

N/A

To check the phosphorylated Src level in 293T-rtTA cell line transduced with Src(Y529F) The results showed the expression of p-Src kinase was induced by 1 μg/mL doxycycline.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human 293T-rtTA cell line

Primary Incubation

1:1000 overnight at 4 degree

Blocking Agent

5% milk

Secondary Incubation

1 hour at room temperature

Tertiary Incubation

1:3000

Detection

ECL

Results Summary

To check the phosphorylated Src level in 293T-rtTA cell line transduced with Src(Y529F) The results showed the expression of p-Src kinase was induced by 1 μg/mL doxycycline.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works well.

The Bad

N/A

The Bottom Line

The antibody works well for western blot and recommended.

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