Abcam
Anti-Alix antibody
ab275377
N/A
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Isolation of murine cardiac extracellular vesicles (EVs) was performed using differential centrifugation. Protein immunoblots of four typical exosomal markers (Alix, Tsg101, CD63 and CD9) for EVs, including increased release of EVs (IR-EVs) and EVs from the sham heart (S-EVs).
Western Blot
Murine EVs
1/1000 dilution 4°C overnight incubation
5% milk
1/2000 dilution room temperature 1 h incubation
Room temperature 10 min incubation
ECL
Isolation of murine cardiac extracellular vesicles (EVs) was performed using differential centrifugation. Protein immunoblots of the typical exosomal marker Alix for EVs, including increased release of EVs (IR-EVs) and EVs from the sham heart (S-EVs).
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FIGURE 1b
The antibody works well as expected.
The antibody works well.