Anti-CD31 Antibody Works Well

September 21, 2022

pathology
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-CD31 antibody

Catalog Number:

ab28364

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: CD31 Antibodies

The protein expression was examined using Western blotting and immunohistochemical (IHC) staining of angiogenesis marker CD31 in tumors obtained from in vivo tumorigenesis experiments. The results showed that USP20 regulated angiogenesis in a β-catenin dependent manner.

Experimental Design and Results Summary

Applications

Western blotting and immunohistochemical (IHC) staining

Sample

Whole cell lysates and tissue slides

Primary Incubation

1/1000 dilution 4°C overnight incubation for Western blotting and 1/200 dilution 4°C overnight incubation for IHC

Blocking Agent

5% milk for Western blotting and 10% goat serum for IHC

Secondary Incubation

1/2000 dilution room temperature 1 h incubation for Western blotting and 1/200 dilution room temperature 1 h incubation for IHC

Tertiary Incubation

Room temperature 10 min incubation for Western blotting room temperature 30 min incubation for IHC

Detection

ECL for Western blotting and DAB for IHC

Results Summary

The protein expression was examined using Western blotting and immunohistochemical (IHC) staining of angiogenesis marker CD31 in tumors obtained from in vivo tumorigenesis experiments. The results showed that USP20 regulated angiogenesis in a β-catenin dependent manner.

DOI or PMID #

https://static-content.springer.com/esm/art%3A10.1038%2Fs41418-018-0138-z/MediaObjects/41418_2018_138_MOESM1_ESM.pdf

Additional Notes

Figure S6E & S6F

Image Gallery

Summary

The Good

The antibody works well as expected.

The Bad

N/A

The Bottom Line

The antibody works.

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