Good Kit for Cytokine Measurement in Media Culture

September 19, 2022

University of Texas Medical Branch Galveston

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD OptEIA Human IL-6 ELISA Set

Catalog Number:

555220

Checking for inflammation in cells associated to pregnancy and infection. Using media from cell culture to measure different types of cytokines.

Experimental Design and Results Summary

Application

Cytokine measurement

Starting Material

Media culture

Tips

Dilution could be needed depending on the sample and the treatment.

Results Summary

Based on the standard curve that comes with the kit, levels of IL-6 can be detected using a plate reader. The values are in pg/mL. The values of the standard curve can be done in duplicates for better performance.

DOI or PMID #

N/A

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Summary

The Good

Easy to use; range of detection is good.

The Bad

None

The Bottom Line

BD OptEIA is a good option for IL-6 detection in culture media. In most of the cases, optimization for dilution is required, since this is a common cytokine (depending on the research field). It is possible to run 20 plates per kit, which is a good number for the price paid.

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