Iba1 Antibody Ideal for Spinal Cord Tissue

September 08, 2022

Houston Methodist

Overall

Quality of Results

Ease-of-Optimization

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Company:

FUJIFILM

Product Name:

anti-Iba1

Catalog Number:

019-19741

Clone Number:

N/A

Info:Supplier Page View Product Specs

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Study role of microglia in spinal cord tissue after contusion injury in rat animal model. Validation of treatment to reduce inflammation by immunofluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Spinal cord tissue

Primary Incubation

1:500, over night, 4 degree Celsius

Blocking Agent

10% goat serum in 0.5% Triton-X100-PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Secondary antibody fluorescence

Results Summary

Ideal for microglia and macrophage detection. Membrane staining including processes and ramification.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works every time.

The Bad

Does not distinguish microglia from macrophages.

The Bottom Line

Ideal for clear staining of microglia/macrophages.

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