Great Anti-IKBa Antibody

August 25, 2022

Surgery
University of Pittsburgh
Research Scientist

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Company:

Cell Signaling Technology

Product Name:

anti-IKBa antibody

Catalog Number:

4814

Clone Number:

N/A

Info:Supplier Page View Product Specs

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BT20 cells were treated with 30 ng/ml TNFalpha for 30 minutes and IKBalpha was examined by Western blot to check the response of NFkB signaling pathway.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BT20

Primary Incubation

1/1000 dilution 4 degrees overnight

Blocking Agent

5% milk

Secondary Incubation

1:1000 dilution

Tertiary Incubation

Room temperature 1 hour

Detection

ECL

Results Summary

BT20 cells were treated with 30 ng/ml TNFalpha for 30 minutes and IKBalpha was examined by Western blot to check the response of NFkB signaling pathway. The results showed that this pathway was activated after TNFalpha treatment.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works.

The Bad

N/A

The Bottom Line

The antibody works.

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