Anti-E-Cadherin Antibody

August 23, 2022

Surgery
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

anti-E-Cadherin antibody

Catalog Number:

3195

Clone Number:

N/A

BT20 cells were treated with 30 ng/ml TNFalpha for 30 minutes. After treatment, E-Cadherin was detected to check if it was involved in the EMT process for drug resistance.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BT20

Primary Incubation

1/1000 dilution 4 degrees overnight

Blocking Agent

5% milk

Secondary Incubation

1:1000 dilution

Tertiary Incubation

Room temperature 1 hour

Detection

ECL

Results Summary

BT20 cells were treated with 30 ng/ml TNFalpha to check how E-cadherin was responded. The results showed that E-cadherin was slightly decreased after treatment.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works.

The Bad

N/A

The Bottom Line

The antibody works well.

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